Hematopoietic progenitor cells as a predictive of CD34+ enumeration prior to peripheral blood stem cells harvesting

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Zefarina Zulkafli
Rapiaah Mustaffa
Shafini Mohammed Yusoff

Keywords

CD34 Count, Hematopoietic Progenitor Cell, Flow Cytometry, Automated Hematology Analyzer, Peripheral Blood Stem Cell Mobilization

Abstract

Background: To date, the CD34+ cell enumeration has relied predominantly on flow cytometry technique. However, flow cytometry is time consuming and operator dependent. The application of the hematopoietic progenitor cells (HPCs) channel in Sysmex XE-2100, a fully automated hematology analyzer offers an alternative approach, which is with minimal sample manipulation and less operator dependent. This study evaluates the utility of HPC counts as a predictive of CD34+ enumeration prior to peripheral blood stem cells harvesting.


Materials and methods: HPC, CD34+, white blood cell (WBC), reticulocytes (retic), immature platelet fraction (IPF) and immature reticulocyte fraction (IRF) were determined in 61 samples from 19 patients with hematological malignancies (15 lymphoma and 4 multiple myeloma patients) at Hospital Universiti Sains Malaysia (Hospital USM) who had received granulocyte-colony stimulating factor (G-CSF) and planned for autologous transplantation.


Results: CD34+ count showed strong and significant correlation with HPC. The receiver operating characteristics (ROC) curve analysis revealed that HPC count > 21.5 x 106 / L can predicts a pre harvest CD34+ count of >20 x 106 / L with sensitivity of 77%, specificity of 64% and area under the curve (AUC) of 0.802.


Conclusion: We concluded that HPC count can be a useful potential parameter in optimizing timing for CD34+ enumeration prior to leukapheresis.

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